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. 2013 Nov 26;42(4):2602–2623. doi: 10.1093/nar/gkt1228

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Synthesis of nm⁵U₃₄ and cmnm⁵U₃₄ is depending on the growth phase. HPLC analysis of total tRNA purified from TH48 (A), TH49 (B), TH69 (C) and IC6629 (D) during the growth cycle. The strains were grown in LBT. The percentage of nucleosides represents the distribution of the peak area of each nucleoside compared to the sum of the peak areas of the two nucleosides considered. Each time point represents the average from two independent experiments. The standard deviations were within ±20%.