Table 3.
Kinetic parameters with respect to chi-
for reactions catalyzed by MnmC, MnmC(o), MnmC(m), and MnmEG
| Reaction | Km (μM) | Vmax (nmoles min−1 mg−1) | kcat (s−1) | kcat/Km (s−1 μM-1) |
|---|---|---|---|---|
| MnmC(FAD) (cmnm5s2U → nm5s2U) | 15.7 ± 3.4 | 457 ± 12 | 0.59 ± 0.02 | 0.038 |
| MnmC(o) domain(FAD) (cmnm5s2U → nm5s2U) | 6.1 ± 2.1 | 486 ± 69 | 0.39 ± 0.05 | 0.064 |
| MnmC(SAM) (nm5s2U → mnm5s2U) | 4.4 ± 1.1 | 365 ± 77 | 0.46 ± 0.10 | 0.105 |
| MnmC(m) domain(SAM) (nm5s2U → mnm5s2U) | 4.2 ± 1.1 | 895 ± 179 | 0.52 ± 0.10 | 0.124 |
| MnmEG(NH4) (s2U → nm5s2U) | 0.6 ± 0.2 | 5.9 ± 0.3 | 0.012 ± 0.001 | 0.020 |
The values are the mean ± SD of a minimum of three independent experiments.