Table 5.
Reprogramming of U34 modification also depends on the tRNA species
Specific tRNAs | Growth Phase | LBTa |
|
---|---|---|---|
cmnm5s2U | nm5s2U | ||
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Exponential | 94 ± 1 | 6 ± 1 |
Stationary | 4 ± 2 | 96 ± 2 | |
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Exponential | 99 ± 1 | 1 ± 1 |
Stationary | 93 ± 2 | 7 ± 2 |
aRelative (%) distribution of the nucleosides in accordance with the growth phase and the tRNA species. Native tRNAs were purified from strain ΔmnmC (IC6010) during the exponential (OD600 ∼0.4) or stationary phase (OD600 ∼3).