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. 2013 Nov 27;42(4):2512–2524. doi: 10.1093/nar/gkt1184

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Idefix silencing is reduced in the dividing germline cysts of the germarium. Immunodetection of Vasa, Aub, Ago3, Piwi, H3K9me³, HP1 and Rhino proteins (white) and GFP (green) in germaria of ovaries expressing the pGgIdas transgene under the control of the act-Gal4 driver. Germaria are oriented with anterior to the top or left. GFP signal is specifically observed in 4-, 8- and 16-cell germline cysts in which Piwi does not accumulate. Experiments were performed on pGgIdas lines as1 and as2.