Figure 10.

VP5 stimulates RNA strand annealing. (A) Schematic illustrations of the HEX-labeled 46-nt RNA strand and the nonlabeled longer complementary 146-nt RNA strand. Asterisk indicates the HEX-labeled strand. (B) Equal amounts (0.1 pmol each) of the two strands were mixed and reacted with increasing amounts of MBP-VP5 for 15 min. Lane 1, the mixture was boiled before being loaded onto the gel as a negative control; lane 2, the two strands were preannealed using a thermal cycler as a positive control; lane 3, the reaction with MBP alone as a negative control; and lanes 4–8, the reactions with 5, 10, 15, 20 and 40 pmol MBP-VP5.