Figure 3.

Monomeric and dimeric MBP-VP5 bind to ssRNA. (A) MBP alone (lane 3) or MBP-VP5 (lane 5) was incubated in a chemical cross-linking buffer containing 0.01% glutaraldehyde (GA) as indicated, and then analyzed via 10% SDS-PAGE, followed by western blots with anti-MBP antibodies as described in ‘Materials and Methods’ section. Lanes 2 and 4, MBP alone and MBP-VP5 in the absence of 0.01% GA. (B) MBP-VP5 was firstly UV cross-linked with HEX-labeled RNA1 probe, and then subjected to chemical cross-linking by incubating with 0.01% GA. The samples were analyzed via 10% SDS-PAGE, followed by scanning using a Typhoon 9200 imager to visualize HEX-labeled RNA probe. The dimeric and monomeric forms of MBP-VP5 are indicated.