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. 2013 Dec 5;42(4):2538–2554. doi: 10.1093/nar/gkt1256

Figure 4.

Figure 4.

VP5 destabilizes RNA helices. Purified MBP-VP5 was incubated with (A) standard RNA helix (R*/R substrate), (B) DNA helix (D*/D substrate), (C) DNA/RNA hybrid helix (D*/R substrate) or (D) RNA/DNA hybrid helix (R*/D substrate) as illustrated in the left panels. Asterisk indicates HEX-labeled strands. The preparations of these destabilizing substrates are indicated in ‘Materials and Methods’ section. Helix substrate (0.1 pmol) was incubated in standard reaction mixtures in the presence or absence of 10 pmol MBP-VP5 as indicated, and the destabilizing activity was determined via gel electrophoresis and scanning on a Typhoon 9200. Lane 1, boiled reaction mixture without protein supplementation; lane 2, reaction mixture without protein supplementation; lane 3, reaction mixture with MBP alone; and lane 4, reaction mixture with MBP-VP5.