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. 2013 Dec 5;42(4):2538–2554. doi: 10.1093/nar/gkt1256

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — VP5 destabilizes stem-loop structured RNA strands. (A) Schematic illustrations of the stem-loop structures of the two complementary 42-nt RNA substrates. HEX labeling is indicated (right). (B–D) The two strands were mixed (0.1 pmol each strand) and reacted in the presence of (B) 40 pmol MBP, (C) 10 pmol MBP-VP5 or (D) 40 pmol MBP-VP5 for the indicated time (lanes 3–8; 1, 2, 4, 8, 10 and 16 min). For (B–D), the mix of the two strands was boiled (lane 1) or preannealed (lane 2) as a negative or positive control. The hybridized and free strands are indicated.