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. 2013 Dec 5;42(4):2708–2724. doi: 10.1093/nar/gkt1271

Figure 6.

Figure 6.

U1-tfs are degraded more rapidly than mature-U1 snRNA is. (A) RNAs were prepared from y18Sn-WT-, y18Sn-ΔSmSL4- or y18SnΔ3′ box-expressing 293T cells at 0, 3 or 6 h after 1 µg/ml actinomycin D (Act.D) treatment, and analyzed by northern blotting with the probe for y18Sn or 5S rRNA (5S) (top). (Bottom) The y-axis shows the staining intensities relative to that of the cells harvested at 0 h. The values indicated are averages (±SD) of three independent experiments. *P < 0.05. **P < 0.01. (B) RNAs were prepared from y18Sn-WT-, y18Sn-U1A3-, y18Sn-ΔSmSL4- or y18Sn-U1A3ΔSmSL4-expressing 293T cells and analyzed by northern blotting with the probes indicated. RAT-tag vector was used as a transfection control.