Table 1.

Equilibrium dissociation constants (K_d) for Trp mutants to A₁₅ and U₆

	A15		U6
E. coli Hfq
WT Hfq S	1.4 nM ± 0.9 a,b	Fold changec	766.0 nM ± 73.7	Fold change
F11W	7.1 nM ± 0.6	5.0	502.7 nM ± 55.5	0.7
R17W	3.7 nM ± 1.5	2.6	1.6 µM ± 0.08	2.1
Y25W	258.2 nM ± 32.7	184.4	836.8 nM ± 62.5	1.1
K31W	1.4 nM ± 0.5	1.0	1123.3 ± 73.0	1.5
Q33W	15.9 nM ± 3.6	11.4	404.7 nM ± 182.4	0.5
F39W	0.59 nM ± 0.35	0.4	2.0 µM ± 0.4	2.6
F42W	0.28 nM ± 0.09	0.2	4.8 µM ± 782.5	6.2
G77W	2.9 nM ± 1.7	2.1	379.9 nM ± 93.2	0.5
Y83W	7.4 nM ± 2.8	5.3	777.9 nM ± 254.1	1.0
Q95W	2.6 nM ± 1.1	1.9	848.3 nM ± 289.4	1.1
S. aureus Hfq
WT Hfq	4.2 nM ± 0.5 d		69.8 nM ± 7.0 d
F25W	11.3 µM ± 5.0	2700	41.9 nM ± 25.1	0.6
Q31W	1.6 µM ± 0.08	386.7	114 nM ± 23.2	1.6

^aEach value is the average of three individual experiments and the standard deviations.

^bTaken from (39).

^cFold change is the ratio of the K_d of the mutant Hfq divided by the K_d of the WT Hfq.

^dTaken from (41). Values listed in bold are all fold increases above 10.0.