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. 2014 Feb 23;12:53. doi: 10.1186/1479-5876-12-53

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Copyright © 2014 Zhao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The peripheral and local expression of sFGL2. Serum sFGL2 presented a fluctuating increase after transplantation, reached the peak at day 10 and gradually fell off eversince (A). Immunohistochemistry staining at 400× magnification showed that sFGL2 positive cells included TECs, interstitial cells and a few in the glomeruli (B). The number of sFGL2 positive cells in the kidney (C) and western blotting (D-E) revealed that there was no significant difference in the expression of sFGL2 between normal renal tissue and that of day 2 post transplantation. sFGL2 in the kidney remarkably increased from day 5, culminated at day 7 and gradually decreased at day 10 and 14. Data are expressed as mean ± SEM (n = 3). Post N: post nephrectomy. *: P < 0.05; **: P < 0.01; ***: P < 0.001.