Table 1.
Primer | Sequence (5’-3’) | Location | Annealing temperature (°C) | Product size (bp) | Reference |
---|---|---|---|---|---|
IntF |
GGCATCCAAGCAGCAAGC |
5’-CS of class 1 integrons |
58 |
varied |
[17] |
IntB |
AAGCAGACTTGACCTGAT |
3’-CS of class 1 integrons |
|||
IntI2L |
GTAGCAAACGAGTGACGAAATG |
intI2 |
58 |
789 |
[17] |
IntI2R |
CACGGATATGCGACAAAAAGGT |
||||
IntI2CaF |
GATAAAAACAGCCTGACCTCTTC |
intI2 |
58 |
2433 |
[17] |
IntI2CaR |
CCCACTTGACATCTCATCAATAC |
3’ region of class 2 integrons |
|||
ST.GYRA1 |
TGTCCGAGATGGCCTGAAGC |
DNA gyrase A |
55 |
470 |
[18] |
ST.GYRA12 |
CGTTGATGACTTCCGTCAG |
||||
mphAF |
AACTGTACGCACTTGC |
macrolide 2’-phosphotransferase |
50 |
837 |
[19] |
mphAR |
GGTACTCTTCGTTACC |
||||
Multiplex 1 |
|
|
|
|
|
B/O_F |
GCGGTCCGGAAAGCCAGAAAAC |
RNAI |
60 |
159 |
[15] |
B/O_R |
TCTGCGTTCCGCCAAGTTCGA |
||||
FIC_F |
GTGAACTGGCAGATGAGGAAGG |
repA2 |
60 |
262 |
[15] |
FIC_R |
TTCTCCTCGTCGCCAAACTAGAT |
||||
A/C_F |
GAGAACCAAAGACAAAGACCTGGA |
repA |
60 |
465 |
[15] |
A/C_R |
ACGACAAACCTGAATTGCCTCCTT |
||||
P_F |
CTATGGCCCTGCAAACGCGCCAGAAA |
iterons |
60 |
534 |
[15] |
P_R |
TCACGCGCCAGGGCGCAGCC |
||||
T_F |
TTGGCCTGTTTGTGCCTAAACCAT |
repA |
60 |
750 |
[15] |
T_R |
CGTTGATTACACTTAGCTTTGGAC |
||||
Multiplex 2 |
|
|
|
|
|
K/B_F |
GCGGTCCGGAAAGCCAGAAAAC |
RNAI |
60 |
160 |
[15] |
K/B_R |
TCTTTCACGAGCCCGCCAAA |
||||
W_F |
CCTAAGAACAACAAAGCCCCCG |
repA |
60 |
242 |
[15] |
W_R |
GGTGCGCGGCATAGAACCGT |
||||
FIIA_F |
CTGTCGTAAGCTGATGGC |
repA |
60 |
270 |
[15] |
FIIA_R |
CTCTGCCACAAACTTCAGC |
||||
FIA_F |
CCATGCTGGTTCTAGAGAAGGTG |
iterons |
60 |
462 |
[15] |
FIA_R |
GTATATCCTTACTGGCTTCCGCAG |
||||
FIB_F |
GGAGTTCTGACACACGATTTTCTG |
repA |
60 |
702 |
[15] |
FIB_R |
CTCCCGTCGCTTCAGGGCATT |
||||
Y_F |
AATTCAAACAACACTGTGCAGCCTG |
repA |
60 |
765 |
[15] |
Y_R |
GCGAGAATGGACGATTACAAAACTTT |
||||
Multiplex 3 |
|
|
|
|
|
I1_F |
CGAAAGCCGGACGGCAGAA |
RNAI |
60 |
139 |
[15] |
I1_R |
TCGTCGTTCCGCCAAGTTCGT |
||||
Frep_F |
TGATCGTTTAAGGAATTTTG |
RNAI/repA |
60 |
270 |
[15] |
Frep_R |
GAAGATCAGTCACACCATCC |
||||
X_F |
AACCTTAGAGGCTATTTAAGTTGCTGAT |
ori γ |
60 |
376 |
[15] |
X_R |
TGAGAGTCAATTTTTATCTCATGTTTTAGC |
||||
HI1_F |
GGAGCGATGGATTACTTCAGTAC |
parA-parB |
60 |
471 |
[15] |
HI1_R |
TGCCGTTTCACCTCGTGAGTA |
||||
N_F |
GTCTAACGAGCTTACCGAAG |
repA |
60 |
559 |
[15] |
N_R |
GTTTCAACTCTGCCAAGTTC |
||||
HI2_F |
TTTCTCCTGAGTCACCTGTTAACAC |
iterons |
60 |
644 |
[15] |
HI2_R |
GGCTCACTACCGTTGTCATCCT |
||||
L/M_F |
GGATGAAAACTATCAGCATCTGAAG |
repA, B, C |
60 |
785 |
[15] |
L/M_R |
CTGCAGGGGCGATTCTTTAGG |
||||
oricolE_F |
GTTCGTGCATACAGTCCA |
ColE plasmids |
60 |
187 |
[16] |
oricolE_R |
GGCGAAACCCGACAGGAC |
||||
IncR_F |
TCGCTTCATTCCTGCTTCAGC |
IncR plasmids |
60 |
251 |
[16] |
IncR_R |
GTGTGCTGTGGTTATGCCTCA |
||||
IncU_F |
TCACGACACAAGCGCAAGGG |
IncU plasmids | 60 | 843 | [16] |
IncU_R | TCATGGTACATCTGGGCGC |