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. 2014 Feb 17;15:26. doi: 10.1186/1471-2202-15-26

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Copyright © 2014 Huang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Eps8 is specifically expressed in GAD67-expressing cells. (A) Doubled-labeled confocal immunofluorescent images showing the colocalization of GAD67 (green) and NeuN (red) in hippocampal area CA1 of GAD67-GFP knock-in mice. (B) Doubled-labeled confocal immunofluorescent images showing the colocalization of GAD67 (green) and Eps8 (red) in hippocampal area CA1 of GAD67-GFP knock-in mice. The merged image indicates that all Eps8-immunopositive cells express GAD67. Arrows point to double-labeled cells. (C) Bar graph showing the percentage of GFP-labeled interneurons that expressed Eps8 in hippocampal area CA1 of GAD67-GFP knock-in mice. Similar results were obtained in 4 mice. Scale bar, 20 μm.