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. 2014 Feb 17;15:26. doi: 10.1186/1471-2202-15-26

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Copyright © 2014 Huang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Eps8 is specifically expressed in calbindin-expressing interneurons. (A) Confocal immunofluorescent image of calbindin-expressing interneurons located in the stratum pyramidale (SP) and stratum radiatum (SR) (left). Confocal immunofluorescent image of immunolabeling for Eps8 in the same section (middle) and the merged image (right) indicates that calbidin-expressing interneurons express Eps8 protein (arrow). (B) Doubled-labeled confocal immunofluorescent images showing that parvalbumin-expressing interneurons in the stratum oriens (SO), SP and SR do not express Eps8 protein. (C) Doubled-labeled confocal immunofluorescent images showing that parvalbumin-expressing interneurons in the SP and SR do not express Eps8 protein. Similar results were obtained in 4 mice. Scale bar, 20 μm.