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. 2014 Feb 13;12:10. doi: 10.1186/1478-811X-12-10

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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G_iα proteins regulate differential activation of Akt, mTORC1 and ERK1/2 by growth factors. (A-F) WT and Gi1/3 DKO MEFs were treated with (A) EGF (100 ng/ml), (B) HB-EGF (100 ng/ml), (C) aFGF (25 ng/ml), (D) bFGF (25 ng/ml), (E) IGF-1 (20 ng/ml), and (F) insulin (1 μg/ml at the indicated time points. p-ERK (T202/Y204), p-AKT (S473), p-S6 (S235/236), or β-actin were detected by immunoblotting (IB) analysis. The experiments were performed more than six times and similar results were obtained. (G) WT and Gi1/3 DKO MEFs were treated with PDGF for 10 minutes (min) at the indicated doses. p-ERK(T202/Y204), p-AKT(S473), p-S6(S235/236), or β-actin were detected by IB. The experiment was repeated three times and similar results were obtained.