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. 2014 Feb 13;12:10. doi: 10.1186/1478-811X-12-10

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Copyright © 2014 Wang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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G_iα proteins regulate differential activation of ERK1/2, Akt and mTORC1 in breast cancer cells in response to EGF, bFGF and IGF-1. MB231 cells were transfected with mock or siRNA duplexes (combination of G_i1α and G_i3α, or G_i1α, G_i2α and G_i3α). Cells were then treated with (A) EGF (100 ng/ml), (B) bFGF (25 ng/ml), or (C) IGF-1 (20 ng/ml) for the indicated time durations. p-AKT (S473), p-ERK (T202/Y204), p-S6 (S235/236), G_i1α (Gi1), G_i2α (Gi2), G_i3α (Gi3) or β-actin were detected by IB. The experiments were repeated two to three times and similar results were obtained.