Figure 6.

Notch accumulates in early endosomal compartments in awd mutant cells. (A) Stage 8 egg chambers were dissected from yw (wild-type; upper panel) or yw; en2.4-Gal4^e22c, UAS-flp/+; FRT^82B/FRT^82B, awd^j2A4 (awd clone, lower panel) females. In wild-type, Notch shows low level punctates that are partially co-localized with Avl. In the awd clone, most, if not all, large Notch-positive vesicles are also Avl-positive. Dashed line marks the awd clone. (B) Surface view of stage 7 egg chamber dissected from a yw; en2.4-Gal4^e22c, UAS-flp/+; FRT^82B/FRT^82B, awd^j2A4 female and stained for Notch extracellular domain peptide (NECD), Awd and DNA. There is accumulation of NECD on the surface of awd mutant clones (empty arrowheads). (C-D) Stage 8 egg chambers were dissected from hs-flp/GbeSu(H)_m8-lacZ; act-Gal4, UAS-GFP/+; FRT^82B, act-Gal80/FRT^82B, awd^j2A4 females. awd mutant clones were identified as GFP-expressing cells. In awd clones, over-accumulated Notch does not co-localize with Rab7 (C) or Rab11 (D). Bars are 20 μm. Avl, avalanche; Awd, abnormal wing disc.