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. 2014 Feb 13;9:3. doi: 10.1186/1749-8104-9-3

Figure 4.

Figure 4

Light induces c-fos expression in melanopsin-expressing retinal ganglion cell (mRGCs) but not melanopsin-expressing horizontal cells (mHCs) as early as Stage 37/38. (A) Double in situ hybridization against opn4m and c-fos on a transverse retinal section from a Stage 42 embryo. Merged image with DAPI staining is also shown. (B) Quantification of the number of c-fos + cells that also express opn4m in the RGC layer (mRGCs) and in the INL (mHCs) expressed as percentages. The number of cells counted is indicated. (C)In situ hybridization against opn4m (Stage 32 and 33/34) (left panels) and double in situ hybridization against opn4m and c-fos (Stage 37/38) (right panels). Left: Transverse sections through the peripheral eye (higher magnification Stage 32 and 33/34) and the brain (Stage 33/34) are shown. mHCs (opn4m+ / c-fos−; arrowhead), mRGCs (opn4m+ / c-fos+; arrow) and RGCs (opn4m− / c-fos+; gray arrowhead) are indicated. Scale bar = 50 μm.