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. 2014 Mar 1;25(5):566–582. doi: 10.1091/mbc.E13-07-0360

FIGURE 1:

FIGURE 1:

Expression of CXCR4ΔCTD in MCF-7 breast carcinoma cells results in up-regulation of cadherin 11 and ZEB-1. (a) Western blot of cadherin 11 and tubulin. Densitometric scans from triplicate assays were quantitated and normalized to the loading control (tubulin). (b) Immunofluorescence staining of cadherin 11. Arrows indicate cadherin 11 localization at cell–cell contacts. Bars, 150 μm. (c) Immunofluorescence staining for colocalization of cadherin 11 and p120 and cadherin 11 and β-catenin in MCF-7 CXCR4ΔCTD and MDA-MB-231 cells. Cells were stained with mouse monoclonal anti-cadherin 11, rabbit polyclonal anti-p120, and rabbit polyclonal β-catenin antibodies and incubated with species-specific Cy3- and Cy5-conjugated secondary antibodies. Overlay images are pseudocolored; red is cadherin 11, and blue is p120 or β-catenin. Image represents a single z-section of 0.28 μm. Insets, enlarged 2× from original images. (d) Western blot of ZEB-1. Densitometric scans from triplicate assays were quantitated and normalized to the loading control (tubulin). (e) Immunofluorescence staining of ZEB-1. Bars, 150 μm.