FIGURE 2:

The cAMP signaling pathway in wild-type and coronin A–deficient cells. (A, B) Vegetative wild-type, coronin A–deficient, and coronin A–deficient cells complemented with coronin A were washed, resuspended in BSS, and seeded at 75,000 cells/well in a 96-well plate format adapted for the xCELLigence measurement. Impedance was measured every 30 s during 22 h and is represented as cell index. (C) Production of cAMP in response to starvation was determined in wild-type, coronin A–deficient, and coronin A–deficient cells complemented with coronin A after 6 h in a BSS suspension. The produced cAMP was determined using the HTRF cAMP assay (Cisbio) as described in Materials and Methods. Data in A and B are presented as a representative experiment out of three independent experiments. Results in C are presented as means ± SD of triplicate determinations of a representative experiment of three independent experiments. **p < 0.001 (t test).