FIGURE 6:

Transition from growth to development induced by conditioned medium in wild-type but not coronin A–deficient cells. (A) Exponentially growing wild-type and coronin A–deficient cells were washed in PBM and seeded into multiwell plates at a density of 5 × 10⁴ cells/cm², incubated in conditioned medium obtained from wild-type or coronin A–deficient starving cells, and imaged over a period of 20 h. The images are taken from a series of frames during the conditioned medium incubation. Bar, 200 μm. See also Supplemental Movies S11 and S12. (B) Cells were prepared as described in A and inoculated in a plastic dish. Images are taken after 6 h of incubation of the cells in conditioned medium. Bar, 100 μm (large pictures), 50 μm (insets).