FIGURE 1:
Lack of Nck enhances PERK activity. (A) PERK activity (pThr-980) in Nck+/+ and Nck−/− MEFs. Equivalent amount of proteins (50 μg) from total cell lysates prepared from three independent culture dishes for each genotype were subjected to immunoblotting with indicated antibodies. (B) Nck+/+ and Nck−/− MEFs were left untreated (lanes 1 and 2) or treated with 1 μM Tg for 10 min (lanes 3–8). Cell lysates (50 μg of protein) from independent culture dishes were analyzed by immunoblotting with the indicated antibodies. Bar charts show ratio of pThr-980 PERK to total PERK in unstressed cells (A) and pThr-980 PERK to total PERK and peIF2αS51 to total eIF2α upon Tg treatment as determined by densitometry (B). Data are means ± SEM (n = 3; *p = 0.004, **p < 0.001, #p = 0.01).