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. 2014 Jan 31;13:18. doi: 10.1186/1476-4598-13-18

Figure 2.

Figure 2

IL-1β upregulates MMP2 and MMP9 expression and activity by activating p38. A: RT-PCR analysis showed that MMP2 and MMP9 mRNA were upregulated in both AGS and MKN-45 cells in response to treatment with IL-1β; this effect was blocked by p38 siRNA or the p38 inhibitor SB202190. B: Quantification of the expression of MMP2 and MMP9 mRNA normalized to GAPDH mRNA. **P < 0.05 vs. scramble siRNA-transfected cells stimulated with IL-1β. △△P < 0.05 vs. untransfected cells stimulated with IL-1β. C: Gel zymography showed that MMP2 and MMP9 activity were upregulated in both AGS and MKN-45 cells in response to treatment with IL-1β; this effect was blocked by p38 siRNA or the p38 inhibitor SB202190. D: Immunocytochemical staining and confocal microscopy confirmed that IL-1β increased activation of p38 (red), and MMP2 and MMP9 (green) expression in MKN-45 GA cells; these effects were blocked by p38 siRNA or the p38 inhibitor SB202190.