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. 2014 Feb 27;10(2):e1004099. doi: 10.1371/journal.pgen.1004099

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Representative spermatocytes in paraffin sections of Sun1^{+/+(Δex10-11)} and Sun1^{−/−(Δex10-11)} testis stained for SYCP3 and KASH5. In the wildtype (A) the expected KASH5 localization at the distal ends of synaptonemal complex axes can clearly be observed. In Sun1^{−/−(Δex10-11)} spermatocytes (A′–A″) the KASH5 signal, although weaker, is also clearly detectable. As seen in the wildtype, distinct KASH5 foci also co-localize with the ends of synaptonemal complex axes. Scale bars 10 µm.