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. 2014 Jan 30;11:8. doi: 10.1186/1743-7075-11-8

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Copyright © 2014 Kowluru et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Oxidative stress is ameliorated in diabetic rats receiving the supplementation. (a) Total ROS levels were quantified in the retina (5-10 μg) using 2 μM of DCHFDA and the fluorescence emitted was measured at 485 nm and 530 nm. (b) Total antioxidant capacity of the retina was quantified by measuring the ability of the retina to inhibit oxidation of ABTS by metmyoglobin using 5-10 μg retina protein. Each sample was measured in duplicate, and the values are represented as mean ± SD of 6–8 rats in each group. *p < 0.05 and #p < 0.05 compared to normal and diabetes respectively.