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Design of the barcode and synthetic random oligonucleotide used to create the SL. The barcode portion was created through PCR amplification, while the synthesized random oligonucleotide was purchased and converted to a double stranded template. Both portions were digested with SfiI endonuclease, ligated, gel purified, and sequenced to produce the SL. Exact sequences used to generate the final synthetic library are provided.
