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Importance of the 3’ end of the primer sequence on amplification bias. The OBV for the 6 bp window of (n = 4,096) was plotted against the OBV of the sequence of the reverse trimer order. For instance the OBV for the 6-mer “CCG AGG” is plotted against the OBV of “AGG CCG”. Although the sequences have the same GC content, the amplification results are not correlated.
