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. 2014 Jan 30;14:10. doi: 10.1186/1472-6750-14-10

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Copyright © 2014 Pan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Observation windows for the 12 N portion of the SL. (A) The 4 bp window includes the nucleotides 4 bp ahead of the primer:template junction; (B) the 6 bp window includes the primer:template interaction; (C) the 8 bp window includes the primer:template interaction and 2 bp ahead of the primer:template junction; and (D) the 10 bp window includes the primer:template interaction and 4 bp ahead of the junction.