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Experimental set-up for the amplification of the SL with two different primer sets. (A) The SL that contains a 12 N random region (blue) serves as the template for all additional amplification experiments; (B) in the amplified background experiment, the forward primer is placed 4 bp upstream of the 12 N region; (C) in the polymerase amplification tests, the forward primer (grey and pink) covers 6 N of the 12 N region.
