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. 2014 Jan 30;14:10. doi: 10.1186/1472-6750-14-10

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Copyright © 2014 Pan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Bias profiles for the SL and amplification experiments. All bias profiles were created by plotting the observed bias value (OBV) for the 4 bp (n = 256) and 6 bp (n = 4,096) windows of observation on a logarithmic scale versus the amplified sequences, which were arranged from low to high GC content in the order of ATCG; (A) the bias observed after amplification with primer set I; (B) after amplification using primer set II with PGT-A DNA polymerase; (C) using primer set II with QTT-A DNA polymerase; (D) using primer set II with QHH-B DNA polymerase.