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. 2014 Jan 30;14:10. doi: 10.1186/1472-6750-14-10

Table 1.

A comparison of the top thirty ranked QTT-A sequences across all amplification experiments

8 bp window sequence Top 30 ranked QTT-A sequences: comparison of rank in other amplification experiments
QTT-A QTT-AR PGT-A PGT-AR QTT-A63 QTT-A65 QTT-A67 QHH-B
GGGGGC GG
 1
 3
 2
 3
 1
 1
 1
 4180
TTGGGC GG
 2
 1
 3
 9
 8
 5
 2
 20382
GGGCCG GG
 3
 12
 5
 2
 5
 3
 3
 606
TGGCCG GG
 4
 5
 4
 4
 6
 6
 6
 3065
GGTCCG GG
 5
 6
 1
 1
 3
 2
 4
 1666
GGGTGC GG
 6
 9
 6
 13
 16
 12
 9
 2377
GTGGGC GG
 7
 11
 10
 5
 9
 7
 11
 1464
TTGCCG GG
 8
 8
 8
 7
 22
 21
 20
 13440
GTGCCG GG
 9
 13
 7
 8
 7
 13
 17
 220
GGGGGC CG
 10
 19
 21
 26
 2
 4
 5
 3950
TGGTGC GG
 11
 18
 9
 14
 37
 28
 19
 8530
TTGGGC CG
 12
 10
 19
 24
 21
 27
 14
 26404
TGCCCG GG
 13
 14
 13
 6
 13
 15
 21
 3370
GGTCCG GC
 14
 17
 11
 12
 18
 18
 23
 3802
GGGGGC CA
 15
 31
 24
 29
 12
 14
 15
 8559
GGGCCG GC
 16
 32
 30
 15
 10
 9
 28
 2255
TGGCCG GC
 17
 15
 15
 16
 26
 23
 22
 5312
TTGTGC GG
 18
 27
 23
 40
 51
 69
 44
 17564
TGTCCG GG
 19
 21
 12
 11
 32
 33
 33
 9598
TTGGGC CA
 20
 16
 34
 35
 58
 51
 47
 29598
GGTGGC GG
 21
 22
 16
 17
 19
 22
 24
 1890
GGGGGC CC
 22
 36
 47
 45
 15
 17
 13
 10961
TTTGGC GG
 23
 33
 32
 22
 57
 71
 39
 24345
GGCCCG GG
 24
 26
 14
 10
 17
 16
 18
 508
TGGGCG GG
 25
 29
 29
 23
 29
 47
 40
 5616
GGGCGC GG
 26
 87
 33
 38
 25
 34
 42
 1337
TTGCCG GC
 27
 23
 31
 32
 46
 52
 65
 18324
TTGGGC GC
 28
 24
 51
 57
 92
 62
 60
 25137
GGGGGC GC
 29
 47
 58
 64
 27
 24
 27
 9051
GGGGGC TG 30 35 55 80 4 8 10 8558

The top thirty sequences of the QTT-A touchdown PCR experiments were ranked and compared to the QTT-A repeat experiment (QTT-AR), the PGT-A and PGT-A repeat experiments (PGT-AR), the QTT-A experiments performed at 63°C, 65°C, and 67°C (QTT-A63, QTT-A65, and QTT-A67), and the QHH-B touchdown PCR amplification experiment.