Table 2.
The 8 bp window motif analyses for several DNA polymerase experiments
|
Top 1% (655 in 65,536) |
8 bp window motif analyses for several DNA polymerase experiments |
|||||||
|---|---|---|---|---|---|---|---|---|
| QTT-A | QTT-AR | PGT-A | PGT-AR | QTT-A63 | QTT-A65 | QTT-A67 | QHH-B | |
|
(GC,CG) motif at 3’ end of primer |
88% |
89% |
90% |
90% |
90% |
91% |
88% |
25% |
|
(GC,CG) motif at 5’ end of 6 N portion of the primer |
8% |
6% |
8% |
11% |
12% |
10% |
8% |
89% |
|
(TC) motif at first 2 bp of runway |
3% |
4% |
3% |
3% |
4% |
4% |
4% |
19% |
|
(CC) motif at first 2 bp of runway |
28% |
26% |
28% |
30% |
23% |
23% |
25% |
30% |
|
(CGC,CCG,GCG, GGC,TGC,TCG) motif at 3’ end of primer |
87% |
88% |
88% |
89% |
90% |
90% |
87% |
22% |
|
(AGC,ACG) motif at 3’ end of primer |
1% |
1% |
1% |
1% |
1% |
1% |
1% |
4% |
|
(ACG) motif at 3’ end of primer |
1% |
1% |
1% |
1% |
1% |
1% |
1% |
2% |
| (AGC) motif at 3’ end of primer | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2% |
The motif percentage breakdown for the top 1% of ranked sequences are shown for several PCR experiments: QTT-A touchdown, QTT-AR, PGT-A, PGT-AR, QTT-A63, QTT-A65, QTT-A67, and the QHH-B touchdown PCR amplification experiment.