Figure 6. Mice with combined CathA/Scpep1 deficiency have reduced ET-1 degradation rate.

Blood plasma, lungs and aorta were collected from WT, Scpep1 ^−/−, CathA^S190A and DD mice 15 min post an intravenous injection of saline or ET-1 in saline (10 nmol/kg BW). The levels of ET-1 peptide were measured by ELISA in lung extracts (A), aorta extracts (B), and in blood plasma extracts (C) from mice injected with ET-1 and blood plasma extracts from mice injected with saline (D). Data are expressed as mean values (±S.E). At least 5 mice were studied for each genotype. * p<0.05 in Welch's modification of two-tailed unpaired t-test.