Figure 4.

ErGPCR knockdown by dsRNA injection blocks larval-pupal transition. (A) Phenotypes after ErGPCR knockdown (dsErGPCR injection into the 5th instar larval hemocoel). Scale bar = 1 cm. (B) Statistical analysis of the phenotype in A. (C) 5–0 h to 6–0 h: 0 h of the 5th instar to 0 h of the 6th instar 0 h; 6–0 h to pupation: 0 h of the 6th instar to pupation. (D) The efficiency of ErGPCR knockdown, analyzed by semi-quantitative RT-PCR. (E) The gene transcripts in the larval epidermis after ErGPCR knockdown were determined by qRT-PCR analysis. Error bars represent the standard deviations of three replicates. Asterisks denote significant differences (*p < 0.05; **p < 0.01, via Student’s t test) based on 30 larval samples with three replicates.