Figure 4. (A) Features and design of the RIM/DS complementation screen. Average lifespan (days) of wildtype (WT), CD2-MYC, CD2-Runx2 and CD2-MYC/Runx2 double transgenic mice, without and with MoMLV infection.

Percentage value indicates lifetime lymphoma incidence. 12 lymphomas from each genotype were analysed by RIM/DS, identifying the indicated number of RISs (total reads in parentheses). (B) Individual RIS in log rank order according to number of reads. Horizontal dotted line represents 100 reads per RIS and was used as a threshold for expanded CISs shown in (d). (C) Total reads in each genotype cohort comprising the CISs around Myc/Mycn/Pvt1 (yellow), Ikzf1 (red), Runx2/Runx3 (purple), around other CISs from the biased CIS set (green) or other RISs (grey). The total numbers of RISs that contribute to the overall read count are indicated outside the pie charts (D) Schematic representation of all RISs with at least 100 reads detected by DS. Each square represents a single RIS, with colour coding as in (c). Expanded RISs not falling within a CIS (presumptive passenger RIS) are not depicted. These analyses illustrate the reduced complexity and greater clonal expansion in MoMLV accelerated CD2-MYC tumours.