Table 2. Strains used.
Name | Strain | Mutation | Reference |
SA564-based strains | |||
WT | PR01 | ΔpyrFE | [21] |
ΔJ1 | PR01-01 | ΔrnjA (SA0940) | [21] |
ΔJ2 | PR01-04 | ΔrnjB (SA1118) | [21] |
ΔJ1ΔJ2 | PR01-17 | ΔrnjA::ermC, ΔrnjB | This work |
J1AGA | PR01-27 | GTGACCATGT to GGCGCCTGCT at position 1068202* | This work |
J2AGA | PR01-37 | ACACGGACATG to AGCAGGCGCCG at position 1268999* | This work |
ΔY | PR01-02 | Δrny | [21] |
ΔcshA | PR01-15 | ΔcshA::kanA Chromosomal region between 2134254 and 2137592* substituted with the kanA gene | This work |
RN4220-based strains | |||
RN4220ΔpyrFE | PR02 | [21] | |
RN4220ΔJ1C | PR02-03 | ΔJ1::CAT194 | This work |
RN4220ΔJ1E | PR02-06 | ΔJ1::ermC | This work |
*) S. aureus N315 nomenclature and chromosomal positions are used.