Table 3. Plasmids and vectors.
| Name | Comment | Reference |
| Vectors for generating allelic replacements | ||
| pRLY-vector series | Range of vectors for allelic replacements | [21] |
| pRLYC9-J1ERYS-1 | Used for generating the RNase J1 deletion in strain PR01-17 | This work |
| pRLYT9-J1ERYS-1 | Used for generating the RNase J1 deletion in strains PR02-06 | This work |
| pRLYE9-J1CATS-1 | Used for generating the RNase J1 deletion in strain PR02-03 | This work |
| pRLYT-J1AGA-1 | Used for generating the J1AGA mutant PR01-27 | This work |
| pRLYT-J2AGA-3 | Used for generating the J2AGA mutant PR01-37 | This work |
| pRLYE-85RL-Kan-1 | Used for generating the cshA deletion mutant PR01-15 | This work |
| Vectors for complementation | ||
| pEB01 | Empty vector Multi-copy shuttle vector for E. coli and S. aureus | [32] |
| pJ1 | Complementation plasmid with RNase J1 pEB01 with chromosomal region 1069181–1066588* | This work |
| pJ2 | Complementation plasmid with RNase J2 pEB01 with chromosomal region 1268514–1270625* | This work |
| pJ1AGA | Complementation plasmid with RNase J1AGASame as pJ1, but with the AGA active site mutation. | This work |
*) Chromosomal positions from S. aureus N315 are used. The RNase J1 gene is in a putative operon with an upstream transcriptional regulator (SA0941), and both genes were included in the pJ1 plasmid, in order to include the promoter and to avoid the problems with RNase J1 expression described by Mäder and coworkers [8].