Table 5. Enrichment of 5′-ends in the RNase J mutants.
Strain | Total # of positionsa | Enrichedb % | Similarc % | Reducedd % |
ΔJ1 | 831 | 20.0 | 73.4 | 6.6 |
ΔJ2 | 905 | 15.8 | 80.0 | 4.2 |
ΔJ1ΔJ2 | 918 | 17.9 | 75.9 | 6.2 |
J1AGA | 830 | 20.6 | 73.4 | 6.0 |
ΔY | 981 | 2.1 | 95.7 | 2.1 |
ΔcshA | 1172 | 4.3 | 91.6 | 4.1 |
) Only positions where both WT and mutant strain exhibited 2 reads or more (normalised with total number reads from the strain) were included.
) Enriched: Positions where the normalised mutant strain exhibits more than 4× the number of reads in the WT (Mutant>4×WT).
) Similar: (WT/4<Mutant<4×WT).
) Reduced: Positions where the normalised mutant strain exhibits less than 4× the number of reads in the WT (WT/4>Mutant).