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. 2014 Feb 27;10(2):e1003975. doi: 10.1371/journal.ppat.1003975

Figure 3. Compared to PMLIII, PMLIV has a higher anti-VSV activity.

Figure 3

(A) U373MG-EV, U373MG-PMLIII or U373MG-PMLIV cells were not infected (-) or infected with VSV at MOIs of 0.2 or 1 for 8 h. Cell extracts were analyzed by Western blotting and revealed by anti-VSV and anti-Actin antibodies. (B) Supernatants from infected U373MG-EV, U373MG-PMLIII or U373MG-PMLIV cells were used for the determination of the virus yields. Mean values and standard deviations of three independent experiments are shown. (C) PMLIV protected cells from VSV-induced cell lysis. U373MG-EV and U373MG-PMLIV cells were left uninfected (-) or infected with VSV at MOIs of 0.2, 1 or 2 for 12 h. The phase contrast picture was acquired using a Nikon Eclipse TS100 microscope and Coolpix MDC lens camera.