Skip to main content
. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: Virology. 1998 Aug 15;248(1):139–147. doi: 10.1006/viro.1998.9254

Fig. 2.

Fig. 2

Levels of CyPA in wild-type and nef-defective HIV-1 particles. Virions produced by transfection of 293T cells were purified by centrifugation through 20% sucrose. The pellets were lysed and subjected to Western blot analysis using aliquots of cytoplasmic extracts normalized for p24 content by ELISA. Proteins were detected with (A) rabbit anti-cyclophilin A; (B) mouse anti-HIV-1 CA, and (C) rabbit anti-Nef; blots were developed using chemiluminiscent detection. Lane 1: wild-type HIV-1, lane 2: wild-type HIV-1 produced in the presence of 10 µM cyclosporin A; lane 3: nef-defective HIV-1, lane 4: env-defective HIV-1; lane 5: env-defective, nef-defective HIV-1.