Fig. 3.

Nef and the CyPA-Gag interaction independently enhance HIV-1 infectivity. Viruses were produced by transfection of 293T cells and were assayed for infectivity using P4-2 cells as targets. Shown are the mean values of triplicate infections, with error bars representing one standard deviation. The paired solid and open bars are the results of independent virus stocks. R7:wild-type HIV-1; R7ΔN:nef-defective HIV-1. R7.221 and ΔN.221 contain a mutation of Gly221 of capsid to Ala; R7.222 and ΔN.222 contain a mutation of Pro222 to Ala.