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Effects of gag mutations of HIV-1 packaging of CyPA. Virions produced by transfection of 293T cells with proviral DNA constructs and were purified by ultracentrifugation through 20% sucrose. Viral pellets were dissolved in lysis buffer, assayed for p24 content by ELISA, and subjected to immunoblot analyses. The blots were probed sequentially with rabbit anti-CyPA (panel A) and mouse anti-CA (panel B), and protein bands were visualized using chemiluminescent detection.
