Fig. 5.

Inhibition of HIV-1 infectivity by CsA correlates with the expression of Nef. Viruses were produced by culturing transfected 293T cells for two days in the presence or absence of 10 µM CsA. Virus stocks were assayed for reverse transcriptase activity and their titers determined by infecting P4 cells. Panel A: shown are the mean values of triplicate infections, with error bars representing one standard deviation. The results are representative of two experiments. Shown in panel B are the ratios of the infectivity values obtained for viruses produced in the absence of CsA to those of viruses produced in the presence of CsA.