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. 2014 Feb 27;10(2):e1003917. doi: 10.1371/journal.ppat.1003917

Figure 7. Gem is involved in HTLV-1-infected cell migration.

Figure 7

(A): Western blot analyses were performed on 70 µg of protein extracts from C91/PL (HTLV-1-infected) lymphocytes transfected either with 75 nM of Gem siRNA or control siRNA. Membranes were probed with anti-Gem (1∶2,000), anti-Tax Tab 172 (1∶500) or anti-β-actin (1∶40,000) antibody. (B): Schematic representation of the protocol used for the cellular migration assays. C91/PL cells were transfected either with 75 nM of Gem siRNA or control siRNA. Twenty-four hours post-transfection, 5×105 cells were collected and loaded on a 5 µm permeable Transwell filter. (C): Cell migration was quantified by flow cytometry. (D): Western blot analyses were performed on 70 µg of cellular extracts from C91/PL transfected either with 75 nM of Gem siRNA or control siRNA. Membranes were probed with anti-Gem (1∶2,000) or anti-β-actin (1∶40,000) antibody. *: significantly different, p = 0.018, Student's t-test.