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. 2014 Feb 27;9(2):e89647. doi: 10.1371/journal.pone.0089647

Table 1. CXCL1/KC prevents the induction of iNOS protein expression in hearts of mice challenged with cardiac-specific α-myosin-heavy chain-derived peptide M7Aα.

Immunization Treatment iNOS+Cardiomyocytes CD68+ CD68+iNOS+
None Saline 1.2±1.0 n.d. n.d.
M7Aα Saline 45.5±12.1* 59.3±9.6 54.8±12.5
M7Aα CXCL1/KC 2.5±2.0 n.d. n.d.

Frequency of positive cells in heart sections from mice immunized with M7Aα in CFA 21 days after the initial immunization. Percentage of cells (±SD) staining for iNOS in cardiomyocytes, or CD68 and iNOS in inflammatory cells, was calculated after counting cells per visual field at magnification of x160 on sections from hearts of 3 mice per group. A minimum 100 cells in at east 10 different visual fields was evaluated per heart. One result representative of 3 independent experiments is shown. * p<0.05 when compared to the other groups. n.d., cells were not detected in sufficient numbers, i.e., there were <100 cells per visual field.