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. 2014 Feb 27;9(2):e89397. doi: 10.1371/journal.pone.0089397

Figure 3. Profound depletion of hematopoietic cells in adult mice carrying an Evi1 exon3 deletion.

Figure 3

(A) Kaplan-Meyer survival curves indicate significant lethality in Vav-iCre; Evi1fl3/fl3 mice, with a median survival of 7.7 weeks (Log rank test, Chi square p value <0.0001). (B) Hemograms for 6 to 9 week-old Vav-iCre; Evi1fl3/fl3 mice. These adult mice displayed leucopenia, severe anemia and thrombocytopenia. Mean ± SEM is indicated. *p<0.05, **p<0.01, ***p<0.001, unpaired t-test. (C) Flow cytometric profiles of bone marrow cells from Vav-iCre/+;Evi1fl3/fl3 and littermate control mice (Evi1fl3/+ or Evi1fl3/fl3). HSC and progenitor cell subpopulations were detected by a combination of markers (KSL: c-Kit+, S: Sca-1+, L: lineage−). We found a significant reduction of cells in Evi1-deleted samples, p = 0.00011 and p = 0.0024, for KSL and KL, respectively (unpaired t-test). (D) Colony forming counts for cells from bone marrow of Vav-iCre;Evi1fl3/fl3 and littermate control mice (Evi1fl3/+ or Evi1fl3/fl3). N = 3 for each group, p = 0.0019 (unpaired t-test). No BFU-E and CFU-Mix colonies were identified.