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. 2014 Feb 27;9(2):e89179. doi: 10.1371/journal.pone.0089179

Table 1. Primer sequences used for RT-PCR.

Gene Symbol Gene Name Forward Reverse
Myhc Smooth muscle-Myosin heavy chain 5′-GAGAATGAGAAGAAAGCCAAGAG-3′ 5′-CATCCAGCTCCCGCTGCAGCT-3′
VSM-actin Smooth muscle alpha-actin 5′- GACACCAGGGAGTGATGGTT-3′ 5′- GTTAGCAAGGTCGGATGCTC-3′
Myocd Myocardin 5'-CTCGGAGTCAGCAGATGGATG-3′ 5′-CCTCACTGTCGGTGGCATAGT-3′
SM22 Smooth muscle protein 22 alpha (SM22α, transgelin). 5′- CAC CTA TCC TCA GCC TCA GC-3′ 5′- TCC AAA GGA CAT TGG CTT CC-3′
Osterix Osterix; Sp7 transcription factor 5'- GTACGGCAAGGCTTCGCATCTGA- 3' 5′-TCAAGTGGTCGCTTCGGGTAAAG -3′
Runx2 Runt-related transcription factor 2 5'- CGG GAATGATGAGAACTACTC-3' 5'- GCG GTCAGAGAACAAACTAGG T -3'
BMP-2 Bone morphogenetic protein 2 5'-AAGGCTTCTTCTTGCTGGTG-3' 5'-GCCTTACCCTCATGATGTCC-3'
Dkk1 Dickkopf 1 5' ACA ACT ACC AGC CCT ACC CTT 3' 5' CCT TCT TGC CCT TTG GTG TGA TA 3'
Lrp5 Low density lipoprotein receptor-related protein 5 5′-TGTGCCACTGGTGAG ATTGACT -3′ 5′- ACGCTGGCAGACAAAGTAGAC -3′
Gsk3β Glycogen synthase kinase 3 beta 5'-AGC ATG AAA GTT AGC AGA GAC 3' 5' TCG ATT CTT AAA TCT CTT GTC C 3'
18S rRNA 18S ribosomal RNA 5′-GTAACCCGTTGAACCCCATT-3′ 5′- CCATCCAATCGGTAGTAGCG-3′