Table 1. Multilocus PCR-RFLP genotyping of Toxoplasma gondii isolates from human and domestic animals in Minas Gerais, Brazil.

PCR-RFLP Genotypea	References	SAG1	5′-3′ SAG2	alt. SAG2	SAG3	BTUB	GRA6	c22-8	c29-2	L358	PK1	Apico	Isolates	Mouse virulenceb
#10 (Type I)	[7]	I	I	I	I	I	I	I	I	I	I	I	RH88
#1 (Type II)	[7]	II or III	II	II	II	II	II	II	II	II	II	II	ME49
#2 (Type III)	[7]	II or III	III	III	III	III	III	III	III	III	III	III	VEG
#6 (Br I)	[5]	I	I	I	III	I	II	u-1	I	I	I	I	CH4	Virulent
													CH5	Virulent
#11 (Br II)	[5]	I	I	II	III	III	III	I	III	I	II	III	D3	Intermediate
													D4	Intermediate
													CH7	Intermediate
													CH9	Intermediate
													CH10	Virulent
													CH11	Intermediate
#8 (Br III)	[5]	I	III	III	III	III	III	II	III	III	III	III	D8	Avirulent
													CH12	Avirulent
#2 (Type III)	[3]	II or III	III	III	III	III	III	III	III	III	III	III	CH6	Intermediate
#19	[5]	I	III	III	III	III	III	I	I	I	u-1	I	CH2	Intermediate
													CH3	Intermediate
#108	[5]	I	I	II	III	III	III	II	I	I	III	I	D2	Intermediate
													D5	Virulent
													SAF	Virulent
													RAR	Virulent
#163	[17]	I	III	III	III	III	III	II	I	III	III	III	CH1	Intermediate
#206	[7]	u-1	I	II	III	III	III	II	III	I	III	I	CH8	Intermediate
													EFP	Intermediate
#226 (New)	This study	I	I	u-1	III	III	III	II	I	III	III	I	D1	Intermediate
#227 (New)	This study	I	I	II	III	III	III	u-1	I	III	III	I	D6	Virulent
#228 (New)	This study	I	III	III	III	III	III	u-1	I	I	III	I	D7	Intermediate
#229 (New)	This study	I	I	II	III	III	II	I	I	I	II	I	EGS	Virulent

u-1 is a new allele that is different from the clonal type I, II and III alleles.

^aAccording to ToxoDB PCR-RFLP Genotype Number.

^bBased on previous studies [3], [10].