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. 2014 Feb 27;9(2):e90206. doi: 10.1371/journal.pone.0090206

Figure 2. GST pull-down of S11-RNase by eEF1A.

Figure 2

(A) Stylar total protein extracts containing an S11-RNase were used for chromatography on glutathione agarose beads in the presence of bacterially expressed GST (lanes 1–3) or with a bacterially expressed S. chacoense eEF1A-GST fusion protein (lanes 4–6). Samples for each experiment are 10% of the column flow-through (FT), the wash (W) and the specific eluate (E). Binding of the eEF1A to the S-RNase and to endogenous pollen actin was assessed by Western blot. (B) Binding of the S-RNase to a Lingulodinium polyedrum eEF1A.