Figure 4. Non-NGF stimulated PC12 cell proliferation on different substrates.

PC12 cells were seeded on 35 mm² poly-L-lysine coated Si₃N₄ surfaces (○) or plastic culture dishes (▵) and counted every 2 days. The cells were cultured in DMEM +10% FBS +10% horse serum. The medium was replaced every 3 days. Values are the mean ± S.E. for twenty samples (Two trials of ten samples each). *, p<0.001 vs. plastic culture dish.